European Plant Embryology Consortium
- Acronym EURO-PEC
- Duration 36
Dolf Weijers NL Wageningen University funded by NWO
Other project participants
Gerd Jürgens DE Max-Planck-Institute for Developmental Biology, Tübingen funded by DFG
Thomas Laux DE University of Freiburg funded by DFG
Michael Nodine AUT Gregor Mendel Institute, Vienna funded by FWF
Ben Scheres NL Wageningen University funded by NWO
- Total Granted budget 1.277.600 €
Embryogenesis lays down the foundations of the plant body on which postembryonic development elaborates in a repetitive manner. The early embryo of Arabidopsis is a highly suitable system to study key developmental regulators and the context in which they act because of the limited number of cells and their predictable division patterns. This collaborative research project (CRP) addresses molecular mechanisms underlying each of four critical differentiation events that occur consecutively in early embryogenesis: (1) differential specification of embryonic identity versus extra-embryonic, (2) generation of epidermis versus inner cells, (3) formation of vascular tissue and ground tissue precursors, and (4) specification of shoot and root identity including their respective stem-cell system. We will use previously identified key regulators of these events as starting points and identify the context in which they act. We will also perform genome-wide approaches, including transcript profiling in mutant embryos and chromatin immunoprecipitation followed by next-generation sequencing to identify binding sites and biologically meaningful target genes of key transcriptional regulators. Furthermore, we will employ independent cell-type specific transcriptome profiling approaches to help define the global molecular landscape of these key differentiation events. Finally, we will characterize microRNA-mediated post-transcriptional control as a novel layer of regulation in early embryogenesis. This collaboration will identify regulatory frameworks governing embryonic cell differentiation events and interactions between them. In addition to the advanced genome-wide approaches, genetic analysis as well as 3D- and live embryo imaging will be used to define the role of newly identified regulators in early embryonic patterning.
The consortium comprises five international partners each with a strong record in studying developmental decisions in the embryo. The partner labs have been instrumental in identifying the key developmental regulators that form the starting point of this CRP. While sharing a strong interest in understanding early plant embryogenesis, all partners study a different key step and have complementary methodological expertise. Findings, materials and technologies will be shared among the partners to create a common basis for dissecting the developmental decisions. Coordination of individual efforts is critical to prevent duplication of work and will enable identification of links between developmental steps. This collaborative project will help to organize and consolidate a competitive European research base for studying the most fundamental building blocks of plant development. This will be the only way forward in generating a comprehensive understanding of the earliest formative events in plant life, and will provide the much-needed basis for rational applications in plant breeding, propagation and biotechnology.